Journal: Scientific Reports

Article Title: Engineering a reporter cell line to mimic the high oligomannose presenting surface immunoglobulin of follicular lymphoma B cells

doi: 10.1038/s41598-020-79862-2

Figure Lengend Snippet: FACS and fluorescence in vivo imaging. ( A ) Fluorescent microscopy of engineered BZ-mCherry cell line shows the clear red fluorescence of the reporter cell line (scale bar = 50um). ( B ) Ectopic murine BZ-mCherrry tumors are clearly visible by fluorescence in vivo imaging taken at Em = 620, Ex = 580, Bin = 4/4, Fnumber = f2, exposure = 0.5 s. ( C ) The potential use of the BZ-mCherry cell line for FACS based screening is also evident by the bright red fluorescence. ( D ) We also see a large shift in the mean fluorescent intensity for the BZ-mCherry cells compared to HEK when incubated with FITC-labeled anti-lambda probe.

Article Snippet: FITC labeled anti-lambda light chain (Cat# NB7551 Novus Biologicals) antibody was diluted 1:2000 ratio and blocked with 1% BSA in PBS buffer for 1 h at room temperature with rocking (50 rpm).

Techniques: Fluorescence, In Vivo Imaging, Microscopy, Incubation, Labeling